Honors Theses

College

James Worth Bagley College of Engineering

Department

Department of Agricultural and Biological Engineering

Degree

Bachelor of Science (B.S.)

Major

Biomedical Engineering

Document Type

Honors Thesis

Abstract

Cotton, having a production value of $7.2 billion in 2017, is one of the most valuable crops in the United States. While not a fiber-producing species, the wild Brazilian tetraploid Gossypium mustelinum may produce a hybrid with exceptionally long fibers if crossed with G. hirsutum, one of the most commonly cultivated species. For the first time, presented here is the global proteome profile of the G. mustelinum leaf which may be used as a reference in future studies. Proteins were extracted from three individual G. mustelinum plants. To maximize protein coverage, two sample preparation techniques were utilized: size-based fractionation by SageELF instrument and complex protein mixture. A total of 3005 proteins were identified by nano-liquid chromatography-tandem mass spectrometry and database search, of which 1550 were unique to SageELF fractionation, 472 were unique to complex mixtures, and 983 were common to both techniques. Functional classification by Gene Ontology (GO) Annotation was performed on the identified proteins. For cellular component categorization, 245 were found in protein-containing complexes and 112 were located in organelles such as plastids and Golgi apparatus. The molecular function GO aspect included 306 oxidoreductases, 80 transferases, 71 hydrolases, and 810 proteins involved in binding. Of the proteins involved in biological processes, 241 pertain to transport, 278 belong to biosynthetic processes, and 121 relate to photosynthesis. A total of 16 GO terms such as extracellular region, nucleoplasm, and vacuoles were assigned to proteins identified in the fractionated samples but not in the complex mixture samples. Additionally, GO terms that were found in both types of samples were more plentiful in the fractionated samples than the complex mixture samples. These findings validate the use of two different sample preparation techniques to obtain a more comprehensive proteome coverage.

Publication Date

4-1-2019

Sponsorship

USDA ARS

First Advisor

Pechanova, Olga

Second Advisor

Pechan, Tibor

Third Advisor

Elder, Anastasia

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