Theses and Dissertations

Holly Evans

Issuing Body

Mississippi State University

Advisor

Nicodemus, Molly

Committee Member

Memili, Erdogan

Committee Member

Jousan, Frank D.

Date of Degree

12-13-2019

Original embargo terms

Worldwide

Document Type

Graduate Thesis - Open Access

Major

Agriculture

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Animal and Dairy Sciences

Abstract

The objectives of this study were to determine fatty acid composition and acrosome status from bull sperm with different freezabilities (n = 12). We hypothesized that lipid fractions had differentiated fatty acid compositions and such differences influence sperm freezability and the sperm acrosome. Fatty acids were extracted from fresh frozen sperm and fractionated by solid-phase extraction. Thirtyour fatty acids were quantified. Saturated fatty acids were predominant, accounting for 71 to 80% of fatty acids in both fractions. Differences in composition between fractions existed (P < 0.001). Branched chain fatty acid concentrations (15 to 18 µg) were almost twice that of polyunsaturated fatty acid concentrations found in the polar fractions (8 to 9 µg; P < 0.001). Sperm with differentiated freezabilities had few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P ≤ 0.011). Analyses of acrosome status of sperm revealed that acrosomes were affected differently among bulls.

URI

https://hdl.handle.net/11668/16418

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