Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Wise, Dwayne
Committee Member
Gordon, Donna
Committee Member
Kent-First, Marijo
Date of Degree
5-2-2009
Document Type
Graduate Thesis - Open Access
Major
Biological Sciences
Degree Name
Master of Science
College
College of Arts and Sciences
Department
Department of Biological Sciences
Abstract
We have used Chinese hamster ovary (CHO)-human hybrid cells containing chromosomes 16, 18, X, and 21 to test the ability of human kinetochores to successfully bind to spindle microtubules and to be distributed to the daughter cells. We have established the intrinsic rate of non-disjunction among these human chromosomes noted above and compared these rates with those in cells presented with mitotic challenges such as taxol, nocodazole, and mitosis with unreplicated genomes (MUG). Cells were grown on culture slides, fixed and processed for immunofluorescence and fluorescence in situ hybridization (FISH). Daughter pairs were identified by staining with anti-á-tubulin to identify midbodies. Human centromere DNA probes were used for FISH in order to test for the successful passage of human kinetochores to daughter cells during anaphase. Our data indicate that different human kinetochores vary in their ability to properly engage the spindle and to be successfully distributed. In addition, mitotic challenges have been shown to affect the rate of non-disjunction. The mechanism of this effect is not yet known.
URI
https://hdl.handle.net/11668/15042
Recommended Citation
Evans, Elizabeth Balconi, "CHO-human hybrid cells as models for human chromosome non-disjunction" (2009). Theses and Dissertations. 1056.
https://scholarsjunction.msstate.edu/td/1056