Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Willard, Scott
Committee Member
Schmidt, Ty
Date of Degree
12-10-2010
Document Type
Graduate Thesis - Open Access
Major
Agricultural Life Sciences (Biochemistry)
Degree Name
Master of Science
College
College of Agriculture and Life Sciences
Department
Department of Biochemistry and Molecular Biology
Abstract
Ethanol fermentation of highly concentrated aflatoxin-contaminated corn (Zea mays) was conducted on a lab scale to determine if aflatoxin concentrated in the distilled ethanol and/or dry distillers grain end-products. Alliquots of fermented mash, distilled ethanol, stillage, and dry distillers grain (DDG) were analyzed via LC-MS/MS and immunoassay detection methods for aflatoxin. Results indicate that aflatoxin does not greatly concentrate during fermentation in the DDGs and is undetectable in distilled ethanol. Addition of binders, MTB-100®, to aflatoxin-contaminated DDGs showed great reduction in aflatoxin concentrations when analyzed via LC-MS/MS. Also, an experiment investigating detoxification of aflatoxin using Clorox® was conducted. Results obtained from LC-MS/MS showed a positive correlation of decreased aflatoxin levels with increasing Clorox® levels following a logarithmic trend.
URI
https://hdl.handle.net/11668/15582
Recommended Citation
Singleterry, Rebecca Burgett, "Comparing detection methods of aflatoxin and exploring aflatoxin decontamination methods" (2010). Theses and Dissertations. 1103.
https://scholarsjunction.msstate.edu/td/1103