Development and Optimization of a Rapid Assay Kit for the Detection of Vibrio Cholerae in Bivalves

Author

Demarcus Rashad Carter

Issuing Body

Mississippi State University

Advisor

Kim, Taejo

Committee Member

Schilling, M. Wes

Committee Member

Silva, Juan L.

Date of Degree

12-13-2014

Document Type

Graduate Thesis - Open Access

Major

Food Science and Technology

Degree Name

Master of Science (M.S.)

College

College of Agriculture and Life Sciences

Department

Department of Food Science, Nutrition and Health Promotion

Abstract

A rapid assay kit for Vibrio cholerae (Vc) was developed to detect and quantify Vc cells in oyster samples within 24 h. The kit, formulated within a two -phase (liquid and solid) 96-well plate, can detect biomarker expression of Vc when the enrichment broth and incubation temperature are optimized. The kit showed 91 % selectivity and 92 % specificity when tested with 23 inclusive Vc and 106 exclusive non-Vc strains. The kit was further optimized using 47 samples of oysters, clams, and soil. There was no significant difference in most probable number between the kit, conventional PCR and BAX PCR regardless of agar heating method (autoclaved vs. boiled). The kit’s limit of detection was below 5 cfu/g. The kit is a reliable method for the detection of V. cholerae in bivalve samples.

URI

https://hdl.handle.net/11668/19037

Recommended Citation

Carter, Demarcus Rashad, "Development and Optimization of a Rapid Assay Kit for the Detection of Vibrio Cholerae in Bivalves" (2014). Theses and Dissertations. 1389.
https://scholarsjunction.msstate.edu/td/1389