Theses and Dissertations

Chan Kyu Kim

Issuing Body

Mississippi State University

Advisor

Singh, Jagdish P.

Committee Member

Monts, David L.

Committee Member

Minerick, Adrienne R.

Committee Member

Yueh, Fang Yu

Committee Member

Pulakat, Lakshmidevi

Other Advisors or Committee Members

Willard, Scott T.

Date of Degree

12-15-2007

Document Type

Dissertation - Open Access

Major

Applied Physics

Degree Name

Doctor of Philosophy

College

James Worth Bagley College of Engineering

Department

Applied Physics Program

Abstract

Laser-induced fluorescence (LIF) has been shown to be potentially useful for identifying microorganisms in real time. It is a selective and sensitive technique because the excitation is performed at one wavelength while the emission is monitored at longer wavelengths so that background from the excitation source can be eliminated. This specialized optical property of LIF can be applied to development of an optical sensor capable of quickly, non-invasively, and quantitatively probing complex biochemical transformations in microorganisms. Various bio-photonic optical fiber sensors based on laser-induced fluorescence (LIF) spectroscopy were developed as diagnostic tools for microorganisms. In the first phase, the enhancement of the sensitivity and selectivity of the optical sensor system focused on diagnosis of human breast cancer cell lines and Azotobacter vinelandii (an aerobic soil-dwelling organism). Autoluorescence spectra from human breast cancer cell lines and Azotobacter vinelandii corresponding to different growth environments were investigated. Then, the study has expanded to include the use of gold nanoparticles for specific DNA detection. The use of gold nanoparticales opens a door into construction of a compact, highly specific, inexpensive and userriendly optical fiber senor for specific DNA detection. An optical fiber laser-induced fluorescence (LIF) sensor based has been developed to detect single-strand (ss) DNA hybridization at the femtomolar level. Effects of various experimental parameters and configuration were investigated in order to optimize sensor performance and miniaturize sensor size.

URI

https://hdl.handle.net/11668/17117

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