Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Allen, Peter J.

Committee Member

Petrie-Hanson, Lora

Committee Member

Mischke, Charles C.

Date of Degree

12-11-2015

Document Type

Graduate Thesis - Open Access

Major

Wildlife and Fisheries Science

Degree Name

Master of Science (M.S.)

College

College of Forest Resources

Department

Department of Wildlife, Fisheries and Aquaculture

Abstract

Aquaculture methods are limited for white crappie (Pomoxis annularis), reducing production potential. Therefore, reproduction methods, including induced spawning, sperm cryopreservation and out-of-season spawning, were developed in tank systems. A two week acclimation period (15°C; 3-5 ppt salinity) was necessary to reduce disease-related mortality. Afterwards, four spawning induction hormones and a control were examined to induce spawning. Luteinizing hormone releasing hormone analogue and salmonid gonadotropin releasing hormone analogue performed the best in terms of spawning success and %ertilization. Sperm cryopreservation was effective using Hanks or Ca2+ree Hanks balanced salt solutions with 10%-methanol or 5%-dimethyl-sulfoxide as a cryoprotectant, frozen at 40°C/min. Out-of-season spawning experiments manipulated photoperiod and temperature over 3-wk (9% spawning success; 11% fertilization) and 6-wk (16% spawning success; 55% fertilization) seasonal shifts. Post-experiment maturation data indicate that females were in an intermediate development stage. These experiments demonstrate the potential of advanced spawning techniques to improve annual production of white crappie.

URI

https://hdl.handle.net/11668/18646

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