Advisor

Allen, Peter J.

Committee Member

Petrie-Hanson, Lora

Committee Member

Mischke, Charles C.

Date of Degree

1-1-2015

Document Type

Graduate Thesis - Open Access

Abstract

Aquaculture methods are limited for white crappie (Pomoxis annularis), reducing production potential. Therefore, reproduction methods, including induced spawning, sperm cryopreservation and out-of-season spawning, were developed in tank systems. A two week acclimation period (15°C; 3-5 ppt salinity) was necessary to reduce disease-related mortality. Afterwards, four spawning induction hormones and a control were examined to induce spawning. Luteinizing hormone releasing hormone analogue and salmonid gonadotropin releasing hormone analogue performed the best in terms of spawning success and %ertilization. Sperm cryopreservation was effective using Hanks or Ca2+ree Hanks balanced salt solutions with 10%-methanol or 5%-dimethyl-sulfoxide as a cryoprotectant, frozen at 40°C/min. Out-of-season spawning experiments manipulated photoperiod and temperature over 3-wk (9% spawning success; 11% fertilization) and 6-wk (16% spawning success; 55% fertilization) seasonal shifts. Post-experiment maturation data indicate that females were in an intermediate development stage. These experiments demonstrate the potential of advanced spawning techniques to improve annual production of white crappie.

URI

https://hdl.handle.net/11668/18646

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