Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Bumgardner, D. Joel
Committee Member
Elder, Steven
Committee Member
Butler, Allen
Committee Member
To, Filip
Committee Member
Gilbert, A. Jerome
Date of Degree
12-13-2008
Document Type
Graduate Thesis - Open Access
Major
Psychology
Degree Name
Master of Science (M.S.)
College
College of Arts and Sciences
Department
Department of Psychology
Abstract
The interaction of osteoclasts and osteoblasts regulates bone density and the maintenance of osseointegration of implants. To investigate osteoblastic response to strain, rat osteosarcoma cells were cultured on titanium plates and subjected to one of three treatments: a neutral control (0 microstrain), tension (1000 microstrain), and compression (1000 microstrain). Treatments were applied via 4 point bending for fifteen minutes each day to a strain of 1mm/m at a sinusoidal frequency of 1Hz. Cells were measured for DNA as indicator of proliferation, alkaline phosphatase as indicator of phenotype and total protein and calcium deposition as indicators of matrix formation. Results indicated that DNA and total protein differed significantly between treatments. The alkaline phosphatase levels and calcification of matrix did not. The differences in the levels of DNA indicate different growth patterns between treatments. The total protein levels imply distinctions in matrix deposition. The alkaline phosphatase and calcium levels did not vary significantly, implying no difference in bone-specificity or calcification.
URI
https://hdl.handle.net/11668/17498
Recommended Citation
Pritchard, Nichol Frances, "Educational Video Impact On College Student Knowledge, Opinions, And Referral Attitudes Regarding Attention-Deficit/Hyperactivity Disorder" (2008). Theses and Dissertations. 1636.
https://scholarsjunction.msstate.edu/td/1636