Title

Effect of occidiofungin on morphogenic transformation of Candida albicans

Advisor

Ambinakudige, Shrinidhi S.

Committee Member

Rodgers III, John C.

Committee Member

Dash, Padmanava

Committee Member

Parajuli, Prem B.

Committee Member

Clary, Renee M.

Other Advisors or Committee Members

Travis, Rick

Date of Degree

8-1-2019

Original embargo terms

||7/17/2022||Visible to MSU Only for 3 years||

Document Type

Graduate Thesis - Open Access

Abstract

Candida albicans is a polymorphic fungus that can grow as yeast (Y) and hypha (H). The Y-H morphological switching is controlled through the MAPK, Cek1p. With the prior work showing that occidiofungin prevents C. albicans from forming hypha when added at the time of Y-H switching, we aim to identify the impact of occidiofungin on signaling events associated with morphological switching specifically looking at Cek1 MAPK cascade. Results from this work have demonstrated that Cek1 MAPK is not required for occidiofungin bioactivity. Further, we report that morphologically switching cells are more sensitive to occidiofungin than their non-switching counterparts. Moreover, later stages of hyphal formation exhibit increased sensitivity towards occidiofungin suggesting occidiofungin targets hyphal initiation and/or elongation process. This work also demonstrates that addition of occidiofungin beyond a discrete time window required for hyphal initiation has minimal effect on hyphae formation and elongation.

URI

https://hdl.handle.net/11668/14509

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