Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Willard, Scott T.
Committee Member
Rhinehart, Justin D.
Committee Member
Ryan, Peter L.
Date of Degree
12-13-2008
Document Type
Graduate Thesis - Open Access
Major
Animal Physiology (Program)
Degree Name
Master of Science (M.S.)
College
College of Agriculture and Life Sciences
Department
Department of Animal and Dairy Sciences
Abstract
The objectives of this study were (1) to determine the optimum in vitro culture conditions for in vitro fertilized bovine embryos among culture methods and (2) to investigate whether bovine fertilization and embryo development would occur in simulated microgravity conditions in vitro. In the first part of this study, the result showed that the microdrop method was the optimum culture method among groups; however, FBS supplementation did not significantly affect the bovine preimplantation embryo development in vitro. In the second part of this study, the result showed that bovine in vitro fertilization did not occur in simulated microgravity conditions. Moreover, none of the presumptive zygotes and 2-8 cell stage embryos were able to develop to further stages, while embryos cultured in standard microdrop method culture conditions developed normally.
URI
https://hdl.handle.net/11668/17099
Recommended Citation
Jung, Songi, "Effects Of Culture Methods And Simulated Microgravity Conditions On Development Of Bovine Embryos Produced In Vitro" (2008). Theses and Dissertations. 1728.
https://scholarsjunction.msstate.edu/td/1728