Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Diehl, Susan

Committee Member

Nicholas, D. Darrel

Committee Member

Prewitt, Lynn M.

Committee Member

Diehl, J. Walter

Date of Degree

8-8-2009

Document Type

Graduate Thesis - Open Access

Major

Forest Products

Degree Name

Master of Science (M.S.)

College

College of Forest Resources

Department

Department of Sustainable Bioproducts

Abstract

Real-time RT-PCR was used to assess the effects of interspecific microbial interactions on the expression of genes associated with lignin peroxidase, manganese peroxidase and alcohol oxidase production during the wood decay process. Expression levels of genes encoding the selected lignolytic enzymes were quantitated in one-, two- and multiple-organism interaction tests with the basidiomycetes Trametes elegans, Phanerochaete chrysosporium, Gloeophyllum sepiarium and Gloeophyllum trabeum. Compression strength loss was measured for each decay sample and correlated with gene expression data for each species. Soil microflora actively producing lignolytic enzymes during wood decay were also assessed and identified using degenerative PCR coupled with denaturing gradient gel electrophoresis, cloning and cycle sequencing. Differential expression was detected in three genes in the two-organism interaction tests: manganese peroxidase in T. elegans interactions, lignin peroxidase A in P. chrysosporium interactions and alcohol oxidase in G. sepiarium interactions. A positive linear correlation was observed between lignin peroxidase A expression and compression strength loss in P. chrysosporium interactions.

URI

https://hdl.handle.net/11668/15468

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