Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Ryan, Peter L.
Committee Member
Jousan, Dean
Committee Member
Crenshaw, Mark
Committee Member
Feugang, Jean M.
Committee Member
Christiansen, David
Date of Degree
4-30-2011
Document Type
Graduate Thesis - Open Access
Major
Animal Physiology (Program)
Degree Name
Master of Science (M.S.)
College
College of Agriculture and Life Sciences
Department
Department of Animal and Dairy Sciences
Abstract
First, a preliminary study was conducted looking for the optimum sperm concentration to be used for analysis with the Computer-Assisted Sperm Analysis (CASA). Results showed that 75x106 sperm cells/mL is the optimum one. Then, the actions of relaxin on sperm motility were evaluated by determining the effect of relaxin on full motility characteristics of spermatozoa during storage, using CASA; then identifying the relaxin receptors on spermatozoa, and finally establishing actions of relaxin in intraspermatic cAMP content. Motile spermatozoa were selected through percoll gradient and incubated for 1 hour with 4 relaxin concentrations at 37°C, during four days. Relaxin affected sperm motility (P<0.05). This action appears associated with the presence of relaxin receptors RXFP1 and RXFP2 that were found in spermatozoa. However, the cAMP levels were not affected by relaxin (P<0.05). This study indicates a beneficial action of relaxin on sperm motility; however, its mechanism of action requires further research.
URI
https://hdl.handle.net/11668/19367
Recommended Citation
Rodríguez Muñoz, Juan Camilo, "Effects of Porcine Relaxin Hormone on Motility Characteristics of Boar Spermatozoa during Storage" (2011). Theses and Dissertations. 1778.
https://scholarsjunction.msstate.edu/td/1778