Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Willeford, Kenneth O.
Committee Member
Peebles, E. David
Committee Member
Heitz, James R.
Committee Member
Boyle, John A.
Committee Member
Salin, Marvin L.
Date of Degree
5-11-2002
Document Type
Dissertation - Open Access
Major
Molecular Biology
Degree Name
Doctor of Philosophy
College
College of Agriculture and Life Sciences
Department
Department of Biochemistry and Molecular Biology
Abstract
The purpose of these studies was to determine if an immunomodulator was present in caprine serum. Controlled studies demonstrated that CSF-I, material fractionated from caprine serum possessed an immunomodulatory compound. Caprine serum was further fractionated into it?s peptidic components and a small contaminant of immunoglobulin G and albumin (Caprine serum fraction - immunomodulator 2, or CSF-I2). This was refined to a three peptidic isolate collectively identified as tri-peptidic immunostimulant or TPI. CSF-I2 does not possess antibacterial capabilities (as typically characteristic of a cationic peptide or defensin), does not contain a level of endotoxin sufficient to promote a pyrogenic response, and its functional ability to improve animal survival after an infectious challenge does not reside with molecular weight components greater than 10 kilodaltons, effectively excluding the immunoglobins, albumin, cytokines, and collectins. CSF-I2 was able to significantly reduce the mortality observed in chickens (from 80% to 13%) infected with Pasteurella multocida (Willeford et al., 2000), in mice (from 83% to 13.3%) infected with Salmonella typhimurium, and in canines (from 50% to 9.8%) diagnosed with parvovirus. CSF-I2 may well prove to provide prophylactic and therapeutic health benefits to humans. CSF-I2 may effectively combat pathogenesis when used as either an adjunct with conventional therapy (e.g., antibiotics) or when provided as the primary medicant.
URI
https://hdl.handle.net/11668/16622
Recommended Citation
Parker, Todd Avery, "Elucidating the Immunoactivity of a Goat Serum Peptide" (2002). Theses and Dissertations. 1859.
https://scholarsjunction.msstate.edu/td/1859