Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Smith, David R.
Committee Member
Bailey, R. Hartford
Committee Member
Erickson, Galen E.
Committee Member
Huston, Carla L.
Committee Member
Wills, Robert W.
Date of Degree
12-8-2017
Original embargo terms
Worldwide
Document Type
Dissertation - Open Access
Major
Veterinary Medical Research
Degree Name
Doctor of Philosophy
College
College of Veterinary Medicine
Department
Veterinary Medical Science Program
Abstract
Enterohemorrhagic Escherichia coli (EHEC) are important foodborne pathogens with a bovine reservoir. For many years, research and regulations have focused on the EHEC serogroup most commonly associated with severe human illness, EHEC O157. However, six additional EHEC serogroups have been identified as important human foodborne pathogens and have been declared adulterants in raw, non-intact ground beef by the United States Department of Agriculture’s Food Safety Inspection Service. Collectively these seven organisms are referred to as EHEC-7. With the addition of these six pathogens, epidemiological studies are needed to estimate the probability for cattle to carry them and to identify risk factors associated with their presence in samples of bovine origin. In addition, the potential for pre-harvest control of EHEC-7 in feedlot cattle, particularly by dietary intervention, is a knowledge gap that needs to be addressed. Finally, detection methods of EHEC-7 have not been validated, and there is no “gold-standard” test. The first study included in this dissertation was a cross-sectional study estimating the prevalence and risk factors associated with hide contamination by EHEC-7 on the hides of market beef cows at slaughter. The second study was a longitudinal analysis of EHEC-7 from fecal samples from cow-calf herds in Mississippi and Nebraska. The third study was a randomized controlled trial evaluating the effects of fiber from distillers grains on the probability to detect EHEC-7 in samples from the rectoanal mucosa of feedlot steers. The fourth study included in this dissertation was a Bayesian latent class analysis estimating the diagnostic performance of three EHEC-7 detection methods, specifically modeling the performance and prevalence of EHEC O157 in fecal samples from beef cow-calf herds.
URI
https://hdl.handle.net/11668/19541
Recommended Citation
Schneider, Liesel J., "Epidemiology of Enterohemorrhagic Escherichia Coli: Detection and Ecology in Beef Cattle Systems" (2017). Theses and Dissertations. 1907.
https://scholarsjunction.msstate.edu/td/1907