A Procedure Using Rotylenchulus Reniformis Nucleotide Sequences to Quantitatively Measure Plant-Parasitic Nematode Infestation Levels using Metagenomic Dna Isolated Directly from Soil

Author

Kurtis C. Showmaker

Issuing Body

Mississippi State University

Advisor

Lawrence, Gary W.

Committee Member

Lu, Shien

Committee Member

Klink, Vincent

Committee Member

Balbalian, Clarissa J.

Date of Degree

12-15-2012

Document Type

Graduate Thesis - Open Access

Major

Agricultural Life Sciences

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Abstract

Molecular diagnostic tests have been developed and utilized to diagnose and to confirm diagnoses of many plant-parasitic nematodes. We evaluate the potential of a qPCR assay to detect and quantify Rotylenchulus reniformis in Mississippi directly from soil. A novel pipeline utilizing multiple databases containing nematode DNA and EST sequences was developed to aid in the selection of R. reniformis primers used in a PCR and qPCR assays. In vitro testing showed that the primers and probes developed from the novel pipeline for the qPCR assays could accurately detect the presence of R. reniformis. Subsequent testing resulted in a trend of increasing observed number of R. reniformis resulting in increasing estimates by qPCR

URI

https://hdl.handle.net/11668/18699

Recommended Citation

Showmaker, Kurtis C., "A Procedure Using Rotylenchulus Reniformis Nucleotide Sequences to Quantitatively Measure Plant-Parasitic Nematode Infestation Levels using Metagenomic Dna Isolated Directly from Soil" (2012). Theses and Dissertations. 233.
https://scholarsjunction.msstate.edu/td/233