Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Gavini, Narasaiah
Committee Member
Pulakat, Lakshmidevi
Committee Member
Peng, Zhaohua
Date of Degree
12-13-2008
Original embargo terms
MSU Only Indefinitely
Document Type
Graduate Thesis - Campus Access Only
Major
Biological Sciences
Degree Name
Master of Science
College
College of Arts and Sciences
Department
Department of Biological Sciences
Abstract
modC is part of a set of genes, modABC, which encode the bacterial molybdate ABC transporter. ModC comprises the ATPase. Of the three structural Mod proteins, ModC has received the least attention, believed to be little different from ATPases of other ABC complexes. However, findings of multiple copies of modC in A. vinelandii and identification of a potential molybdate-binding domain in the C-terminal of ModC may point to a more complex role. ORF10 is one of three copies of modC in A. vinelandii; atypically, ORF10 is not found with genes encoding the other ABC transporter components but is instead found as part of the nif gene cluster, encoding molybdenum nitrogenase. The role of ORF10 is investigated here via sequence analysis and comparison of growth of a ORF10- A. vinelandii strain with wild type growth. Findings imply ORF10 optimizes growth under conditions requiring nitrogenixation, specifically under those conditions where molybdate-availability is limited.
URI
https://hdl.handle.net/11668/17464
Recommended Citation
Shivaji, Sangeetha, "Functional Analysis of a ModC Homolog in the Azotobacter Vinelandii Nif-Gene Cluster" (2008). Theses and Dissertations. 2345.
https://scholarsjunction.msstate.edu/td/2345