Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Lu, Shi-En

Committee Member

Smith, James

Committee Member

Sabanadzovic, Sead

Date of Degree

12-9-2011

Document Type

Graduate Thesis - Open Access

Major

Entomology

Degree Name

Master of Science (M.S.)

College

College of Agriculture and Life Sciences

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Abstract

he 56-kb ocfC gene cluster is required for occidiofungin production by Burkholderia contaminans strain MS14. However, the function of the ocfC gene remains unknown. Sequence analysis showed the putative protein encoded by ocfC shares significant identities to glycosyltransferase. Plate bioassays showed that occidiofungin production by the ocfC mutant (ocfC::nptII) was significantly reduced as compared with strain MS14. The occidiofungin produced by the ocfC mutant is xyloseree, which suggests the ocfC gene encodes a xylosyltransferase to add a xylose to the peptide backbone. Single variant optimization of culture condition and medium compositions was also performed in this study. The results indicated that occidiofungin production was promoted with higher cell density inoculum, additional casamino acid, xylose, urea, zinc ions, and at pH 5. The findings have provided insights into development of pharmaceutical drug and agricultural biofungicide.

URI

https://hdl.handle.net/11668/17011

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