Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Sparks, Darrell L., Jr.
Committee Member
Brown, Ashli
Committee Member
Hernandez, Rafael A.
Date of Degree
12-15-2012
Document Type
Graduate Thesis - Open Access
Major
Biochemistry
Degree Name
Master of Science (M.S.)
College
College of Agriculture and Life Sciences
Department
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology
Abstract
The growth of Rhodotorula glutinis in sweet sorghum juice in three levels of three factors of temperature, carbon to nitrogen ratio and pH was evaluated. Accompanying of this growth was the generation of lipids converted to fatty acid methyl ester (FAMEs) and carotenoids. The optimized condition for maximum biomass and carotenoid accumulation was determined to be at 25C, pH of 5.5 and carbon to nitrogen ratio of 10. This condition yielded 22.7 g/L biomass with specific growth rate of 0.213 hr-1. At this condition the carotenoids generation was also maximum with 2.6 mg/gram biomass, comprising of torularhodin, beta-carotene and torulene. The accumulation of lipids following generation of biodiesel was highest at same temperature and pH but carbon to nitrogen ratio of 70, generating 96.3 mg of FAMEs/gram of biomass containing methyl ester of oleic acid, linoleic acid, palmitic acid, stearic acid and linolenic acid.
URI
https://hdl.handle.net/11668/19079
Recommended Citation
Revellame, Miriam Llanto, "Generation of Biodiesel and Carotenoids from Rhodotorula Glutinis using Sweet Sorghum Juice" (2012). Theses and Dissertations. 2389.
https://scholarsjunction.msstate.edu/td/2389