Theses and Dissertations

Peng Deng

Issuing Body

Mississippi State University

Advisor

Lu, Shien

Committee Member

Smith, James L.

Committee Member

Thornton, Justin A.

Committee Member

Baird, Richard E.

Committee Member

Sabanadzovic, Sead

Date of Degree

5-7-2016

Document Type

Dissertation - Open Access

Major

Entomology and Plant Pathology

Degree Name

Doctor of Philosophy (Ph.D)

College

College of Agriculture and Life Sciences

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Abstract

Burkholderia contaminans MS14 shows excellent antimicrobial activities against a wide range of pathogens. Complete sequence analysis reveals that the MS14 genome harbors multiple gene loci that contribute to its antimicrobial activities and lacks key virulence features commonly found in pathogenic Burkholderia species. A mutagenesis study identified the genes required for MS14 antibacterial activities and gene expression profiling targeted a polyketide synthase (PKS) gene cluster. Site-specific mutagenesis confirmed the PKS gene cluster is directly related to MS14 antibacterial activities and the PKS gene product is predicted to be the MS14 antibacterial compound. Strain UFB2 isolated from Mississippi shows significant antifungal and antibacterial activities. UFB2 was classified to be Pseudomonas chlororaphis and its complete genome sequence was reported in this study. Green house trails showed P. chlororaphis strain UFB2 could efficiently reduce the disease severity of bacterial canker of tomato, by significantly inhibiting the growth of the pathogen Clavibacter michiganensis subsp. michiganensis. The research findings of B. contaminans MS14 and P. chlororaphis UFB2 have provided insights into the development of MS14 antibacterial compound for agricultural application and potential use of strain UFB2 as a biocontrol agent.

URI

https://hdl.handle.net/11668/17601

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