Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Reichert, Nancy A.

Committee Member

Baldwin, Brian S.

Committee Member

Klink, Vincent.

Date of Degree

5-6-2017

Document Type

Graduate Thesis - Open Access

Major

Microbiology

Degree Name

Master of Science (M.S.)

College

College of Arts and Sciences

Department

Department of Biological Sciences

Abstract

Arundinaria gigantea, a North American bamboo that historically grew in vast canebrakes, is now considered a critical component of an endangered ecosystem. Expressing self-incompatibility, restoration efforts must ensure genetic diversity within canebrakes for viable seed production. DNA fingerprinting methods were developed using 20 simple sequence repeat (SSR) markers and two sequence-characterized amplified region (SCAR) markers. Among 18 markers able to amplify rivercane DNA via polymerase chain reaction (PCR), 10 were demonstrated to be polymorphic within rivercane. Markers could distinguish rivercane among and between canebrakes and could discern full-sibling seedlings. The mostly-infertile Mississippi canebrakes of rivercane were determined to contain 46% genetic diversity within canebrakes and an average of 1.436 effective alleles. In contrast, the fertile North Carolina canebrakes contained 99% genetic diversity within canebrakes and an average of 6.435 effective alleles. Therefore, theoretically, at least seven distinct genotypes were needed for a healthy, viable rivercane brake.

URI

https://hdl.handle.net/11668/16881

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