Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Gordon, Donna M.
Committee Member
Stewart Jr., James A.
Committee Member
Sepehrifar, Mohammad
Date of Degree
5-6-2017
Original embargo terms
MSU Only Indefinitely
Document Type
Graduate Thesis - Campus Access Only
Major
Biological Sciences
Degree Name
Master of Science
College
College of Arts and Sciences
Department
Department of Biological Sciences
Abstract
In S. cerevisiae, the accumulation of dysfunctional mitochondria activates a retrograde signal that is mediated through multiple cytosolic regulators. Central to activation is the cytosolic regulator Rtg2p which through its interaction with Mks1p, promotes the nuclear translocation of Rtg1p/3p. Nuclear localized Rtg1p/3p promotes transcription of target genes. Prior work has shown Rtg2p interaction with Mks1p is required for downstream signaling, however the Mks1p binding site within Rtg2p is unknown. To identify this motif, random mutations were generated in RTG2 and a red-white screening strategy was used to assess 14,001 clones. Sequence analysis identified four mutants with amino acid mutations in the carboxy-terminus of Rtg2p that gave rise to defects in CIT2 transcription and loss of Mks1p interaction. Relative to RTG2, all mutants had reduced Rtg2p protein half-lives. Together these results suggest that the carboxy-terminal domain of Rtg2p is essential for retrograde signaling as it may contain the Mks1p binding site.
URI
https://hdl.handle.net/11668/17608
Recommended Citation
Jiang, Jian, "Identification of Rtg2P Functional Domain Involved in Retrograde Signaling in Saccharomyces Cerevisiae" (2017). Theses and Dissertations. 2555.
https://scholarsjunction.msstate.edu/td/2555