Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Feugang, Jean M.N. Nguekam
Committee Member
Liao, Shengfa
Committee Member
Crenshaw, Mark
Committee Member
Wills, W. Robert
Date of Degree
8-11-2017
Document Type
Graduate Thesis - Open Access
Major
Agricultural and Life Sciences
Degree Name
Master of Science (M.S.)
College
College of Agriculture and Life Sciences
Department
Department of Animal and Dairy Sciences
Abstract
Semen contain a heterogeneous population of viable and non-viable (damaged) spermatozoa. Proportions of non-viable spermatozoa interfere with male fertility, with available techniques unable to selectively remove prior to breeding. Nanobiotechnology may allow removal, enriching semen with high quality spermatozoa for improved productivity. Here, we applied double nanopurification with boar semen using functionalized magnetic nanoparticles. Non-viable and viable spermatozoa were magnetically separated and verified through various microscopy imaging. Viable (nanopurified) spermatozoa showed no additional damages. Nanopurification did not interfere with sperm motility and viability, with beneficial effects on motion parameters. Nanopurified spermatozoa maintained fertility following insemination, with resulting offspring indicating no impaired growth or health performance. Pork quality was unaffected showing comparable characteristics to the control. In summary, the use of magnetic nanopurification in boar spermatozoa showed sperm viability and fertility improvements with successful offspring performance. This study shows promise for large-scale commercial applications to enhance male fertility and offspring performance.
URI
https://hdl.handle.net/11668/20649
Recommended Citation
Durfey, Casey Lynn, "Impact of Nanotechnology-Based Semen Purification on Reproduction of Gilts and Developmental Performance of Offspring" (2017). Theses and Dissertations. 2599.
https://scholarsjunction.msstate.edu/td/2599