Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Bumgardner, Joel D.
Committee Member
Carr, Russell L.
Committee Member
Cooper, Robert C., Jr.
Committee Member
Elder, Steven H.
Date of Degree
12-11-2004
Document Type
Graduate Thesis - Open Access
Major
Biomedical Engineering
Degree Name
Master of Science
College
James Worth Bagley College of Engineering
Department
Department of Agricultural and Biological Engineering
Abstract
Bone and bone cells have been shown to respond to mechanical forces placed upon them. Particularly, strain plays an important role in osteogenic differentiation of marrow cells around artificial implants in bone. These strains, depending on their magnitude, duration, and repetition, can alter the proliferation and matrix synthesis of osteoblasts. To test how strain parameters influence osteoblast behavior, a four-point bending apparatus was used to impose cyclic strain on osteogenic stem cells isolated from rats and seeded on titanium plates. Cells were stimulated at 1 Hz for 15 minutes daily and compared to an unstrained control. Stimulation occurred at two magnitudes: 400 and 1000 micro-strain, and three levels of repetition: one, three, and five consecutive days. DNA, protein, alkaline phosphatase, and calcium levels were measured to determine the proliferation and matrix synthesis activity of the cells. No statistically significant effect was found for the tested parameters under these conditions.
URI
https://hdl.handle.net/11668/19185
Recommended Citation
Smith, Daniel Henlee, "Imposing Cyclic Strain on Osteogenic Stem Cells: The Effects of Strain Levels and Repetition of Cyclic Strain in an Implant Environment" (2004). Theses and Dissertations. 2638.
https://scholarsjunction.msstate.edu/td/2638