Theses and Dissertations

Issuing Body

Mississippi State University


Bumgardner, Joel D.

Committee Member

Carr, Russell L.

Committee Member

Cooper, Robert C., Jr.

Committee Member

Elder, Steven H.

Date of Degree


Document Type

Graduate Thesis - Open Access


Biomedical Engineering

Degree Name

Master of Science


James Worth Bagley College of Engineering


Department of Agricultural and Biological Engineering


Bone and bone cells have been shown to respond to mechanical forces placed upon them. Particularly, strain plays an important role in osteogenic differentiation of marrow cells around artificial implants in bone. These strains, depending on their magnitude, duration, and repetition, can alter the proliferation and matrix synthesis of osteoblasts. To test how strain parameters influence osteoblast behavior, a four-point bending apparatus was used to impose cyclic strain on osteogenic stem cells isolated from rats and seeded on titanium plates. Cells were stimulated at 1 Hz for 15 minutes daily and compared to an unstrained control. Stimulation occurred at two magnitudes: 400 and 1000 micro-strain, and three levels of repetition: one, three, and five consecutive days. DNA, protein, alkaline phosphatase, and calcium levels were measured to determine the proliferation and matrix synthesis activity of the cells. No statistically significant effect was found for the tested parameters under these conditions.