Theses and Dissertations
In Vitro and In Vivo Assessment of Platelet Function in Healthy Dogs during Low-Dose Aspirin Therapy
Issuing Body
Mississippi State University
Advisor
Thomason, John M.
Committee Member
Mackin, Andrew J.
Committee Member
Bulla, Camilo
Committee Member
Langston, V. Cory
Date of Degree
8-15-2014
Document Type
Graduate Thesis - Open Access
Major
Veterinary Medical Science
Degree Name
Master of Science (M.S.)
College
College of Veterinary Medicine
Department
Veterinary Medical Science Program
Abstract
Low-dose aspirin therapy in dogs inconsistently inhibits platelet function, termed ‘aspirin resistance’. There are no established diagnostic tests that can predict aspirin resistance in dogs prior to therapy. Platelet function was evaluated in healthy dogs prior to and during low-dose aspirin therapy using turbidimetric and impedance aggregometry, PFA-100, and urine 11-dehydro-thromboxane-B2 concentration. Following a washout, platelet-rich plasma from the dogs was incubated with aspirin and evaluated via turbidimetric aggregometry. After aspirin, the majority of dogs were classified as ‘aspirin responders’ with 81% responding after 7 days. Platelet dysfunction was not consistent in all dogs at all times. Compared to turbidimetric, impedance and PFA-100 results were inconsistent when run concurrently, suggesting turbidimetric is the preferred technique. There was poor agreement between in vitro aspirin incubation and all other tests. Unlike in people, platelet function in dogs is consistently inhibited by aspirin incubation, making this a poor technique for predicting aspirin resistance.
URI
https://hdl.handle.net/11668/20048
Recommended Citation
Haines, Jillian Marie, "In Vitro and In Vivo Assessment of Platelet Function in Healthy Dogs during Low-Dose Aspirin Therapy" (2014). Theses and Dissertations. 2668.
https://scholarsjunction.msstate.edu/td/2668