Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Shan, Xueyan

Committee Member

Ma, Din-Pow

Committee Member

DuBien, Janice

Committee Member

Williams, W. Paul

Date of Degree

8-10-2018

Document Type

Graduate Thesis - Open Access

Major

Agricultural Life Sciences (Biochemistry)

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Abstract

Present throughout all classes of life, antimicrobial peptides (AMPs) confer defense against bacteria, viruses, fungi, and insects. Identifying maize AMPs would provide breeders with a new defense resource. Here, the investigation of maize AMPs is reported. The distribution of AMPs within a panel of ten Mississippi maize inbred lines with varying resistance to Lepidoptera larvae feeding and Aspergillus infection is explored to characterize their observed resistances. Homology data-mining with two comprehensive AMP databases revealed 88 unique maize AMP protein sequences across 81 genes in the MaizeGDB B73 genome assembly. AMP-related polymorphic sites were identified using genomic primers. Analyses with qRT-PCR revealed 8 differentially expressed maize AMP genes. Computational 3D models of AMPs are limited, and models of these eight maize AMP genes were predicted. Two-dimensional electrophoresis gels were used to contrast protein profiles of inbreds with varying resistance to identify regions related to AMPs and other defense-related protein.

URI

https://hdl.handle.net/11668/19972

Comments

antimicrobial peptides

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