Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Rogers, E. Rudy
Committee Member
Minerick, R. Adrienne
Committee Member
Hill, J. Priscilla
Committee Member
French, W. Todd
Date of Degree
5-2-2009
Document Type
Graduate Thesis - Open Access
Major
Chemical Engineering
Degree Name
Master of Science
College
James Worth Bagley College of Engineering
Department
Dave C. Swalm School of Chemical Engineering
Abstract
Bacillus subtilis capable of producing surfactin was cultured to evaluate effects of microbial cell mass on natural gas hydrate formation, dissociation, and stability characteristics. The direct molecular influences of microbial cell wall polymers inhibited gas hydrate formation significantly, decreased hydrate formation rates, and increased dissociation rates. Upon the introduction of bentonite, significant synergy was observed in the system in the form of a catalytic effect. Microbes cultured from seafloor seawater-saturated sediments collected from Mississippi Canyon 118 (MC-118) produced similar effects and generalized the observed trends. MC-118 cultures also produced biosurfactant in several culture media, which was shown to catalyze natural gas hydrate formation in porous media. Microorganisms inhabit gas hydrate macrostructures and consume hydrocarbons and other substrates from within. Sulfate reduction and anaerobic hydrocarbon oxidation occurred within gas hydrate during incubations with MC-118 indigenous consortia. A mathematical model was developed to explore the diffusion-reaction implications in massive seafloor gas hydrates.
URI
https://hdl.handle.net/11668/15153
Recommended Citation
Radich, James Gregory, "Laboratory and theoretical investigations of direct and indirect microbial influences on seafloor gas hydrates" (2009). Theses and Dissertations. 2909.
https://scholarsjunction.msstate.edu/td/2909
Comments
gas hydrate||microorganisms||mathematical modeling