Listeria Monocytogenes Response to Sublethal Sodium Hypochlorite Induced Oxidative Stress on its Biofilm Forming Ability and Antibiotic Resistance

Author

Mohit Bansal

Issuing Body

Mississippi State University

Advisor

Kiess, Aaron S.

Committee Member

Sharma, Chander Shekhar

Committee Member

Nannapaneni, Ramakrishna

Date of Degree

12-8-2017

Document Type

Graduate Thesis - Open Access

Major

Agriculture

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Poultry Science

Abstract

Listeria monocytogenes response to oxidative stress by sublethal sodium hypochlorite was investigated in this study. Continuous exposure of sublethal chlorine influenced biofilm formation and stress adaptation (homologous and heterologous) in L. monocytogenes. The biofilm forming ability of oxidative stress adapted and control cells were investigated on polystyrene surface at 22°C and 37°C. The oxidative stress adapted cells were found to form less biofilm in the presence of chlorine (p < 0.10) when compared to non-treated control cells at both the temperatures. In addition, the biofilm forming ability of L. monocytogenes was reduced significantly at higher sublethal chlorine concentrations (p < 0.10). In conclusion, oxidative stress adapted L. monocytogenes has developed tolerance to chlorine and some of the antibiotics. However, oxidative stress those cells did demonstrate an antibiofilm effect. This demonstrates that oxidative stress reduces L. monocytogenes biofilm formation but can also increase antibiotic resistance.

URI

https://hdl.handle.net/11668/18624

Comments

Listeria monocyotgenes||oxidative stress||chlorine||biofilm

Recommended Citation

Bansal, Mohit, "Listeria Monocytogenes Response to Sublethal Sodium Hypochlorite Induced Oxidative Stress on its Biofilm Forming Ability and Antibiotic Resistance" (2017). Theses and Dissertations. 2960.
https://scholarsjunction.msstate.edu/td/2960