Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Wardlaw, Jennifer L.
Committee Member
McLaughlin, Ron M., Jr.
Committee Member
Frondoza, Carmelita G.
Date of Degree
12-15-2012
Document Type
Graduate Thesis - Open Access
Major
Veterinary Medical Science
Degree Name
Master of Science
College
College of Veterinary Medicine
Department
Veterinary Medical Science Program
Abstract
Little research has focused on the involvement of oxidative stress as it relates to the pathophysiology of osteoarthritis (OA); while inflammation has been extensively studied. The present study evaluates the ability to modulate the response of canine chondrocytes to both inflammation and oxidative stress in an in-vitro model. Chondrocytes were incubated and then stimulated to under-go oxidative stress by using hydrogen peroxide or inflammation using interleukin-1 beta and tumor necrosis factor alpha. For inhibition of oxidative stress an antioxidant, N-acetyl-cysteine, was used prior to induction with hydrogen peroxide in a subset of chondrocytes. Measures of oxidative stress were superoxide dismutase and reduced glutathione. Prostaglandin E2 was used as a measurement of inflammation. Chondrocytes responded appropriately to both oxidative stress and inflammation. The antioxidant N-acetyl-cysteine provided adequate protection against oxidative stress. Oxidative stress and inflammation should be considered to play a role in the pathophysiology of canine OA.
URI
https://hdl.handle.net/11668/19699
Recommended Citation
Dycus, David L., "Modulation of Inflammation and Oxidative Stress in Canine Chondrocytes" (2012). Theses and Dissertations. 3175.
https://scholarsjunction.msstate.edu/td/3175
Comments
canine||chondrocyte||oxidative stress||inflammation||osteoarthritis