Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Diehl, Susan V.

Committee Member

Diehl, Walter

Committee Member

Prewitt, M. Lynn

Committee Member

Nicholas, Darrel

Date of Degree

8-9-2008

Document Type

Graduate Thesis - Open Access

Major

Forest Products

Degree Name

Master of Science

College

College of Forest Resources

Department

Department of Forest Products

Abstract

This study focused on isolating important wood decay fungi from two field sites located in Harrison County, MS, and Oktibbeha County, MS. Southern Yellow Pine samples of various types and treatments including: Cu8, CuOm, ACQ, PCP, proprietary organic biocide, and un-treated were collected, and fungal isolates were cultured. DNA was extracted from isolated fungal cultures and amplified by polymerase chain reaction (PCR). The internal transcribe spacer (ITS) region was sequenced, and fungal cultures were identified by comparison to sequences on GenBank using BLAST. A total of 68 fungal isolates were recovered and successfully identified from 196 samples. Thirteen basidiomycete isolates were identified, with Veluticeps fimbriata occurring most frequently. The white-rot ascomycete, Daldinia fissa was also common. Two sequence-specific oligonucleotide probes (SSOP) were designed using Lasergene® PrimerSelect software. Unsuccessful attempts were made to attach poly (dT) tails to the probes in order to cross link the probes to nylon membranes.

URI

https://hdl.handle.net/11668/19363

Comments

BLAST||GenBank||ITS||polymerase chain reaction||DNA

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