Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Thornton, Justin A.
Committee Member
Jordan, Heather
Committee Member
Seo, Ken Seok
Date of Degree
5-1-2020
Original embargo terms
Worldwide
Document Type
Graduate Thesis - Open Access
Major
Biological Sciences
Degree Name
Master of Science
College
College of Arts and Sciences
Department
Department of Biological Sciences
Abstract
Streptococcus pneumoniae is a significant human pathogen and the leading cause of community-acquired pneumonia and acute otitis media. One of the primary defense mechanisms of the human immune system against pneumococcal infection involves granule-mediated killing of bacterial cells by neutrophils. While this mechanism has previously been shown to kill about half of pneumococci in vitro, we hypothesized that some pneumococcal strains have evolved to be more resistant to this granule-mediated killing. Clinical isolates demonstrated a varying range of sensitivity to neutrophil granules. Additionally, we established that the absence of the capsule may affect sensitivity as unencapsulated isolates showed a higher average survival than encapsulated isolates. Finally, pneumococcal surface protease HtrA was found to potentially serve as a protective factor as many knockouts were more sensitive than the wildtypes, recombinant HtrA protected wildtype TIGR4, and a resistant isolate showed higher htrA expression levels than sensitive isolates.
URI
https://hdl.handle.net/11668/16948
Recommended Citation
Jackson, James Howard, "Pneumococcal resistance to granule-mediated killing by human neutrophils" (2020). Theses and Dissertations. 3553.
https://scholarsjunction.msstate.edu/td/3553
Comments
Pneumococcus||Neutrophils||Granules||Resistance||Capsule||HtrA