Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Archer, Todd M
Committee Member
Pinchuk, Lesya M
Committee Member
Mackin, Andrew J
Committee Member
Thomason, John M
Committee Member
Grace, Sharon F
Date of Degree
5-1-2020
Original embargo terms
Worldwide
Document Type
Graduate Thesis - Open Access
Major
Veterinary Medicine Research
Degree Name
Master of Science
College
College of Veterinary Medicine
Department
Department of Clinical Sciences
Abstract
Existing pharmacokinetic monitoring tools for cyclosporine fail to correlate with clinical response. In dogs, pharmacodynamic monitoring of nuclear factor of activated T cell (NFAT) regulated cytokines is thought to provide a better overall evaluation of the immune response to cyclosporine than blood levels; however, such monitoring tools are not available in cats. In this study, we designed and optimized a protocol for maximal T lymphocyte stimulation in cats. This is the first step in the development of a pharmacodynamic monitoring tool for cyclosporine in cats based on expression of NFAT-regulated cytokines. We also confirmed that cyclosporine has anti-lymphocytic properties in cats, and we were the first to document induction of apoptosis by cyclosporine in cats. Differences in individual patient response to cyclosporine may be influenced by apoptotic response of lymphocytes to cyclosporine. Additional studies are required to optimize and validate polymerase chain reaction monitoring of NFAT-regulated cytokines for cyclosporine-mediated immunosuppression.
URI
https://hdl.handle.net/11668/16495
Sponsorship
This work was supported by a grant from the Mississippi State University College of Veterinary Medicine Office of Research and Graduate Studies.
Recommended Citation
Cridge, Harry, "Preliminary investigations into pharmacodynamic monitoring of cyclosporine in cats" (2020). Theses and Dissertations. 3621.
https://scholarsjunction.msstate.edu/td/3621
Comments
cyclosporine||feline||pharmacodynamic monitoring