Author

Harry Cridge

Advisor

Archer, Todd M

Committee Member

Pinchuk, Lesya M

Committee Member

Mackin, Andrew J

Committee Member

Thomason, John M

Committee Member

Grace, Sharon F

Date of Degree

5-1-2020

Original embargo terms

Visible to MSU only for 1 year||forever||10000-01-01

Document Type

Graduate Thesis - Open Access

Major

Veterinary Medicine Research

Degree Name

Master of Science

College

College of Veterinary Medicine

Department

Department of Clinical Sciences

Abstract

Existing pharmacokinetic monitoring tools for cyclosporine fail to correlate with clinical response. In dogs, pharmacodynamic monitoring of nuclear factor of activated T cell (NFAT) regulated cytokines is thought to provide a better overall evaluation of the immune response to cyclosporine than blood levels; however, such monitoring tools are not available in cats. In this study, we designed and optimized a protocol for maximal T lymphocyte stimulation in cats. This is the first step in the development of a pharmacodynamic monitoring tool for cyclosporine in cats based on expression of NFAT-regulated cytokines. We also confirmed that cyclosporine has anti-lymphocytic properties in cats, and we were the first to document induction of apoptosis by cyclosporine in cats. Differences in individual patient response to cyclosporine may be influenced by apoptotic response of lymphocytes to cyclosporine. Additional studies are required to optimize and validate polymerase chain reaction monitoring of NFAT-regulated cytokines for cyclosporine-mediated immunosuppression.

URI

https://hdl.handle.net/11668/16495

Sponsorship

This work was supported by a grant from the Mississippi State University College of Veterinary Medicine Office of Research and Graduate Studies.

Comments

cyclosporine||feline||pharmacodynamic monitoring

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