Simpson, Chartrisa LaShan
Walters, Keisha B.
Howell, George Eli, III
Date of Degree
Graduate Thesis - Open Access
Master of Science
James Worth Bagley College of Engineering
Department of Agricultural and Biological Engineering
Premature death from cardiovascular disease is especially high in patients with chronic kidney disease (CKD). Vascular calcification is a potent risk factor for developing cardiac-related morbidity and mortality and is especially prominent within the CKD population. Deficiencies in serum levels of fetuin-A as well as inadequate production of matrix Gla protein (MGP) correlate inversely with the extent of vascular calcification and time spent on dialysis. Fetuin-A is a well-known systematic regulator of bone metabolism and MGP is a local antagonist of bone forming proteins. To meet the clinical need of at-risk patients prone to cardiac-related mortality. We propose a targeted protein therapy to treat arteriosclerotic arteries. The focus of this thesis was to characterize the binding interactions of fetuin-A with calcium mineral in a simulated body fluid and to study the in vitro effects of a fetuin vitamin K2 co-therapy on the prevention of calcification of vascular smooth muscle cells.
Cunningham, Janice L, "Protein Therapy for the Treatment of Vascular Calcification in Patients with End-Stage Renal Disease" (2015). Theses and Dissertations MSU. 3668.