Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Allen, Peter J.

Committee Member

Colvin, Michael E.

Committee Member

Mischke, Charles C.

Date of Degree

12-14-2018

Document Type

Graduate Thesis - Open Access

Major

Wildlife, Fisheries and Aquaculture

Degree Name

Master of Science

College

College of Forest Resources

Department

Department of Wildlife, Fisheries and Aquaculture

Abstract

White Crappie (Pomoxis annularis) and Black Crappie (P. nigromaculatus) are popular North American gamefish; however, frequent fluctuations in year class strength present a management challenge for recreational fisheries. Intensive aquaculture production has the potential to address this challenge through controlled hatchery reproduction for supplemental stocking, but further study is needed to refine and optimize techniques. Therefore, this study evaluated the effects of hormone injection timing on latency period and spawning success, examined effective cryopreservation techniques for black-stripe Black Crappie sperm (a preferred hatchery phenotype), and compared simulated spring duration on out-of-season spawning success. Latency period for White Crappie did not depend on the diel time of gonadotrophin-releasing hormone injection. Cryopreservation of black-stripe Black Crappie sperm and subsequent fertilization of White Crappie eggs was more effective using 5% dimethyl-sulfoxide than 10% methanol. A longer duration at final spring spawning conditions (3 vs. 2 weeks) increased egg fertilization in out-of-season spawning experiments.

URI

https://hdl.handle.net/11668/19495

Comments

Crappie||White Crappie||Black Crappie||latency period||GnRHa||sperm cryopreservation||out-of-season spawning

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