Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Diehl, Susan
Committee Member
Jones, P. David
Committee Member
Willeford, Ken
Date of Degree
12-9-2011
Document Type
Graduate Thesis - Open Access
Major
Forest Products
Degree Name
Master of Science
College
College of Forest Resources
Department
Department of Forest Products
Abstract
To gain a better understanding of the mechanisms by which the white-rot decay fungus Phanerochaete chrysosporium regulates selective delignification versus simultaneous decay, differential gene expression of its two key enzymes were measured over the course of aspen and birch wood decay tests. The type of decay was determined by differential staining and scanning electron microscopy. Real-time qPCR assessed gene expression of four lignin peroxidase genes and two manganese peroxidase genes at each stage of decay. Differential expression was significant in the mnp2 gene between aspen and birch decay tests during incipient decay; abundant expression of mnp2 in aspen corresponded to early initiation of selective delignification, whereas birch underwent initial simultaneous decay in the absence of abundant mnp2 expression. The lipC gene was the most abundantly expressed lip gene at all time points in both wood species and likely plays an important role in regulating wood decay.
URI
https://hdl.handle.net/11668/17082
Recommended Citation
Parker, Leslie Anne, "Regulation Of Selective Delignification In The White-Rot Decay Fungus Phanerochaete Chrysosporium" (2011). Theses and Dissertations. 3757.
https://scholarsjunction.msstate.edu/td/3757
Comments
selective delignification||regulation||manganese peroxidase||lignin peroxidase||white-rot fungi||gene expression||Phanerochaete chrysosporium