Salmonella enterica serovar enteritidis requires the type three secretion system-1/2 to invade/survive in chicken oviduct epithelial cells and to modulate innate immune responses

Author

Shuhui Li

Issuing Body

Mississippi State University

Advisor

Zhang, Shuping

Date of Degree

5-3-2008

Document Type

Graduate Thesis - Open Access

Major

Veterinary Medical Science

Degree Name

Master of Science

Degree Name

Doctor of Philosophy (Ph.D)

College

College of Veterinary Medicine

College

College of Veterinary Medicine

Department

Veterinary Medical Science Program

Abstract

Contaminated poultry and egg products are major sources of Salmonella enterica serovar Enteritidis (S. enteritidis, SE) infections in humans. Colonization of SE in chicken reproductive tract results in the production of contaminated commercial shell-eggs and fertilized hatchery eggs. The complex pathogen-host interactions during SE colonization of chicken reproductive tract are largely unknown. This study was aimed at determining the pathogenic roles of the type three secretion systems (TTSS-1 and TTSS-2) in SE infection of chicken oviduct epithelial cells (COEC). A series of SE strains carrying mutations in the genes encoding structure or effector proteins of TTSS-1 and TTSS-2 were constructed. The invasiveness and intracellular survival rate of each SE strain as well as the host innate immune responses induced by the infections were evaluated. The results demonstrate that both TTSS-1 and TTSS-2 are required by SE to invade COEC which involve genes encoding effector proteins SipA, SopB, SopE2, and PipB. In addition to their involvement in host cell invasion, sipA and sipB are also necessary for the survival or replication of SE inside COEC. Inactivation of TTSS-2 genes (ssaV and pipB) resulted in an enhanced bacterial proliferation inside COEC. The data from this study also show that SE infection triggers pro-inflammatory responses in COEC and TTSS-1 is involved in the expression of iNOS and IL-8, a CXC chemokine. TTSS-1 and TTSS-2 are not necessary for induction of K203, MIP-1β, and IL-10 or suppression of TGF-β3 in COEC.

URI

https://hdl.handle.net/11668/14991

Comments

pro-inflammatory responses||chicken oviduct epithelial cells||TTSS||Salmonella enterica serovar enteritidis

Recommended Citation

Li, Shuhui, "Salmonella enterica serovar enteritidis requires the type three secretion system-1/2 to invade/survive in chicken oviduct epithelial cells and to modulate innate immune responses" (2008). Theses and Dissertations. 3851.
https://scholarsjunction.msstate.edu/td/3851