Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Silva, L. Juan
Committee Member
White, H. Charles
Committee Member
Schilling, W. Mark
Committee Member
Watson, H. Vance
Committee Member
Mikel, B. William
Date of Degree
12-9-2006
Document Type
Graduate Thesis - Open Access
Major
Food Science, Nutrition and Health Promotion
Degree Name
Master of Science
College
College of Agriculture and Life Sciences
Department
Department of Food Science, Nutrition and Health Promotion
Abstract
Attachment strength of Listeria spp. and their InlA expression was assessed. Listeria monocytogenes 19111 exhibited the strongest attachment strength with L. monocytogenes 19115, L. grayi, L. innocua, and L. monocytogenes 7644 being the weakest. InlA expression was not detected in silver stained SDS gels but was detected in Western blotting images. Internalin A was only detected in protein extracts of L. monocytogenes 19111 and 7644 with band intensities of 50.1 and 2.5 pixels, respectively. Greater InlA expression was correlated with higher attachment strength in L. monocytogenes 19111. Listeria monocytogenes 19115 did not express InlA but it had a stronger attachment than L. monocytogenes 7644 which demonstrated InlA expression. Intensity of InlA expressed in L. monocytogenes 19111 increased when temperature increased from 10 to 40 oC, and L. monocytogenes 7644 only expressed InlA at 40 oC. It was also determined that InlA was more expressed in nutrient-rich media than in nutrient-poor media.
URI
https://hdl.handle.net/11668/15566
Recommended Citation
Chen, Bang-Yuan, "SDS-PAGE and western blotting analyses of Internalin A in Listeria monocytogenes and Listeria spp" (2006). Theses and Dissertations. 3871.
https://scholarsjunction.msstate.edu/td/3871
Comments
Internalin A||Listeria||Attachment strength