Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Lawrence, Mark L.
Committee Member
Karsi, Attila
Committee Member
Perkins, Andy D.
Date of Degree
5-17-2014
Document Type
Graduate Thesis - Open Access
Major
Veterinary Medicine
Degree Name
Master of Science
College
College of Veterinary Medicine
Department
Veterinary Medical Science Program
Abstract
Flavobacterium columnare is a Gram negative fish pathogen that causes columnaris disease, which infects populations of wild and cultured fish species. However, pathogenic mechanisms of F. columnare are largely unknown. The purpose of this research is to obtain the complete sequence of the F. columnare ATCC 49512 genome to advance pathogenesis research and increase our understanding of this pathogen. To accomplish this, genome sequencing by using Sanger and 454 sequencing was conducted. The sequences were assembled, gaps were filled, and the circular genome was autoannotated. The F. columnare genome size is 3.2 Mb and AT rich (68.5% AT). It contains 2,882 predicted proteins, 71 tRNA genes and five ribosomal RNA operons. More than half (57.1%) of the open reading frames have assigned function, which included chondroitin AC lyase, proteases, collagenases, and genes involved in biofilm formation, secretion systems, iron acquisition, and gliding motility.
URI
https://hdl.handle.net/11668/17687
Recommended Citation
Tekedar, Hasan Cihad, "Sequencing and Analysis of the Flavobacterium Columnare ATCC 49512 Genome" (2014). Theses and Dissertations. 3923.
https://scholarsjunction.msstate.edu/td/3923
Comments
Flavobacterium columnare||flavobacterium||genome sequencing||pathogenesis||Genome||vrirulence