Advisor

Lawrence, Mark L.

Committee Member

Karsi, Attila

Committee Member

Perkins, Andy D.

Date of Degree

1-1-2014

Document Type

Graduate Thesis - Open Access

Major

Veterinary Medicine

Degree Name

Master of Science

College

College of Veterinary Medicine

Abstract

Flavobacterium columnare is a Gram negative fish pathogen that causes columnaris disease, which infects populations of wild and cultured fish species. However, pathogenic mechanisms of F. columnare are largely unknown. The purpose of this research is to obtain the complete sequence of the F. columnare ATCC 49512 genome to advance pathogenesis research and increase our understanding of this pathogen. To accomplish this, genome sequencing by using Sanger and 454 sequencing was conducted. The sequences were assembled, gaps were filled, and the circular genome was autoannotated. The F. columnare genome size is 3.2 Mb and AT rich (68.5% AT). It contains 2,882 predicted proteins, 71 tRNA genes and five ribosomal RNA operons. More than half (57.1%) of the open reading frames have assigned function, which included chondroitin AC lyase, proteases, collagenases, and genes involved in biofilm formation, secretion systems, iron acquisition, and gliding motility.

URI

https://hdl.handle.net/11668/17687

Comments

Flavobacterium columnare||flavobacterium||genome sequencing||pathogenesis||Genome||vrirulence

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