Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Bailey, R. Hart
Committee Member
Brooks, John P.
Committee Member
Coble, Keith H.
Committee Member
Hall, Michael E.
Committee Member
Donaldson, Janet R.
Date of Degree
5-11-2013
Document Type
Dissertation - Open Access
Major
Veterinary Medical Science
Degree Name
Doctor of Philosophy
College
College of Veterinary Medicine
Department
Veterinary Medical Science Program
Abstract
The increase in production farming, also known as concentrated animal feeding operations (CAFOs), garners more investigations on the implications to public health regarding the disposal of the wastes of food production animals. In addition to the vast amount of animal manure produced, human biosolids is another waste residual that must be managed. The research focus was the sustainability of foodborne pathogens in waste products and the variables that manipulate these environments such as moisture, temperature, organic matter and time. The first study was designed to analyze spatial differences in microbial populations in broiler litter by investigating the relationship of intra-house location, age of flock, bedding moisture, and seasonality. Antibiogram profiles of selected isolates were explored to determine if antibiotic resistant bacteria are common in these environments and if multiple class resistance is present. These findings provided insight into new targets that may reduce zoonotic bacteria that are problematic from a food safety prospective as well as nuisance bacteria that threaten broiler health. The second study was designed to establish current decay rates of viral and bacterial pathogens when seeded in various waste residuals and the effects soil type and application method have on those rates. Decay rates were established by standard culture and molecular methods, such as qPCR. A comparison of both derived inactivation rates were analyzed to determine if these methods were significantly different. Both cultural and molecular methods have limitation and advantages, and the argument that both are useful and needed is asserted. The decay rates associated with each method were used to simulate a one-time exposure to a land application site to assess the microbial risk of Salmonella using a Quantitative Microbial Risk Assessment model.
URI
https://hdl.handle.net/11668/19283
Recommended Citation
Roberts, Brandy Nicole, "Spatial and Temporal Distribution of Microbial Pathogens in Poultry Litter and the Development of Microbial Inactivation Constants in Waste Application" (2013). Theses and Dissertations. 4005.
https://scholarsjunction.msstate.edu/td/4005
Comments
Foodborne pathogens||Waste Management||Salmonella