Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Pradhan, Prabhakar
Committee Member
Ariunbold, Gombojav
Committee Member
Arnoldus, Hendrik F.
Date of Degree
11-25-2020
Document Type
Graduate Thesis - Open Access
Major
Physics
Degree Name
Master of Science
College
College of Arts and Sciences
Department
Department of Physics and Astronomy
Abstract
Optical microscopy is the oldest form of microscopy that has been visually aiding scientific research. In our research, I have reported here two such optical microscopy techniques for two different projects. In the first project, we re-developed an instrumentation of a cost-effective, high-performing, single-molecular localization super-resolution microscopy setup that breaks the diffraction limitation barrier. Then we use a stochastic image capturing technique to capture the best precision image positions of gold nanoparticles. In our second project, we apply confocal microscopy technique to image DNA molecular nanoscale structural alterations of chromatin in cell nuclei of gut tissues caused by total body irradiation (TBI). We then quantify these alterations using a light localization technique called inverse participation ratio (IPR) using the confocal micrographs of the sample. Our results show radiation causes reduction and saturation of DNA spatial mass density fluctuations that were observed for different durations of post-irradiation.
URI
https://hdl.handle.net/11668/20831
Recommended Citation
Hasan, Mehedi, "Super-resolution microscopy development for the detection of nano-structures and confocal study of the structural damage in gut cell nuclei due to total body irradiation" (2020). Theses and Dissertations. 4153.
https://scholarsjunction.msstate.edu/td/4153
Comments
Super Resolution||Single molecule localization Microscopy||Confocal Microscopy||Structural disorder||Radiation