Author

Mehedi Hasan

Advisor

Pradhan, Prabhakar

Committee Member

Ariunbold, Gombojav

Committee Member

Arnoldus, Hendrik F.

Date of Degree

12-1-2020

Document Type

Graduate Thesis - Open Access

Major

Physics

Degree Name

Master of Science

College

College of Arts and Sciences

Department

Department of Physics and Astronomy

Abstract

Optical microscopy is the oldest form of microscopy that has been visually aiding scientific research. In our research, I have reported here two such optical microscopy techniques for two different projects. In the first project, we re-developed an instrumentation of a cost-effective, high-performing, single-molecular localization super-resolution microscopy setup that breaks the diffraction limitation barrier. Then we use a stochastic image capturing technique to capture the best precision image positions of gold nanoparticles. In our second project, we apply confocal microscopy technique to image DNA molecular nanoscale structural alterations of chromatin in cell nuclei of gut tissues caused by total body irradiation (TBI). We then quantify these alterations using a light localization technique called inverse participation ratio (IPR) using the confocal micrographs of the sample. Our results show radiation causes reduction and saturation of DNA spatial mass density fluctuations that were observed for different durations of post-irradiation.

URI

https://hdl.handle.net/11668/20831

Comments

Super Resolution||Single molecule localization Microscopy||Confocal Microscopy||Structural disorder||Radiation

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