Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Zhang, Dongmao
Committee Member
Foster, Stephen
Committee Member
Saebø, Svein
Date of Degree
12-9-2011
Document Type
Graduate Thesis - Open Access
Major
Chemistry
Degree Name
Master of Science
College
College of Arts and Sciences
Department
Department of Chemistry
Abstract
Malondialdehyde (MDA) is the commonly accepted biomarker of lipid peroxidation. We reported the surface-enhanced Raman (SERS) detection of MDA using Thiobarbituric acid (TBA) as a molecular probe. The lowest concentration of HPLC purified TBA-MDA adduct that can be determined with reasonable signal to noise ratio is 0.45 nM. The specificity of the SERS technique has been demonstrated by comparing the SERS spectrum of TBA-MDA adduct with other TBA-aldehyde adducts. As a small organosulfur compound, TBA exhibits tremendous structural complexity. Discussed in this thesis is the drastic pH and concentration dependence of TBA SERS spectral features. To understand the origins of the TBA SERS spectral variations, UV-Vis spectra of TBA were also acquired under same experimental conditions of SERS. Density function calculations (DFT) were performed for different TBA tautomers with different charge states to facilitate the SERS spectral interpretation, allowing us to speculate the type of tautomers dominating the nanoparticle surfaces.
URI
https://hdl.handle.net/11668/18698
Recommended Citation
Haputhanthri, Pravindya Rukshani, "Surface-Enhanced Raman Spectroscopy of Thiobarbituric Acid (TBA) and TBA Reactive Compounds" (2011). Theses and Dissertations. 4161.
https://scholarsjunction.msstate.edu/td/4161
Comments
Surface-enhanced Raman spectroscopy||Malondialdehyde||Thiobarbituric acid reactive compounds||Thiobarbituric acid