Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Chevalier, David J.
Committee Member
Klink, Vincent P.
Committee Member
Gordon, Donna M.
Date of Degree
8-11-2012
Document Type
Graduate Thesis - Open Access
Major
Biological Sciences
Degree Name
Master of Science
College
College of Arts and Sciences
Department
Department of Biological Sciences
Abstract
The involvement of several genes in DNA repair in animals and bacteria, and the induced expression of At3G02400 by Mitomycin C, suggest that this gene is involved in DNA repair in plants. I have tested this hypothesis by studying the regulation of its expression and identifying the function of this gene. To study the expression of this gene, I used a reporter line and bio-computing tools. To identify the function of the gene, I have isolated two T-DNA mutants and screened Targeting Induced Local Lesions in Genomes (TILLING) mutants. T-DNA mutagenesis is the process of inserting transfer DNA into the genome, which disrupts the genetic expression. My study showed that Camptothecin, Bleomycin, and Mitomycin C induced the expression of At3G02400. In addition to this, the promoter truncation studies identified a 50 bp region just upstream of the start codon of At3G02400 that maybe required for the induction of expression.
URI
https://hdl.handle.net/11668/20180
Recommended Citation
Gray, Will, "The Analysis of the Expression and Phenotype of the at3g02400 Gene in Arabidopsis Thaliana" (2012). Theses and Dissertations. 4263.
https://scholarsjunction.msstate.edu/td/4263
Comments
At3G02400||Arabidopsis thaliana