Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Memili, Erdogan
Committee Member
Nicodemus, Molly C.
Committee Member
Jousan, F. Dean
Date of Degree
8-7-2020
Original embargo terms
Visible to MSU only for 1 year
Document Type
Graduate Thesis - Open Access
Major
Agriculture (Animal Science)
Degree Name
Master of Science
College
College of Agriculture and Life Sciences
Department
Department of Animal and Dairy Sciences
Abstract
The objective of this study was to uncover molecular and cellular dynamics in spermatozoa from Holstein bulls with Good (11 bulls) and Poor (5 bulls) cryotolerance. Post-thaw sperm kinetics, membrane integrity, and DNA fragmentation were assessed. Data was analyzed using principal component analysis. The spermatozoa from Good bulls had a higher number of cells with intact membranes (P = 0.029), nonragmented DNA (P = 0.018), post-thaw viability (P < 0.001) in comparison to those of the Poor bulls. The Good bulls also had a faster average path velocity (VAP; P = 0.017), straight-line velocity (VSL; P = 0.036), a greater distance average path (DAP; P = 0.006) and distance straight line (DSL; P = 0.011). No differences were found in total antioxidant capacity (TAC), number of live cells or other kinetic parameters between spermatozoa from Good and Poor groups, showing that no one characteristic can be used to determine cryotolerance.
URI
https://hdl.handle.net/11668/18453
Recommended Citation
Gilmore, Alicia, "The molecular and cellular dynamics of Holstein bull spermatozoa associated with cryotolerance" (2020). Theses and Dissertations. 4567.
https://scholarsjunction.msstate.edu/td/4567