Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Karsi, Attila.
Committee Member
Peterson, Daniel.
Committee Member
Thornton, Justin A.
Committee Member
Lawrence, Mark L.
Date of Degree
5-6-2017
Document Type
Dissertation - Open Access
Major
Veterinary Medical Science
Degree Name
Doctor of Philosophy
College
College of Veterinary Medicine
Department
Veterinary Medical Science Program
Abstract
Edwardsiella ictaluri is an intracellular Gram-negative pathogen, causing enteric septicemia of catfish (ESC). Universal stress proteins (USP) are important in bacterial virulence, but the role of USPs in E. ictaluri virulence is not explored yet. Our aim was to analyze gene expression of 13 usp (usp01-usp13) and 7 USP-interacting genes (groEL, groES, dnaK, dnaJ, clpB, grpE, and ppGpp) under low pH, H2O2, catfish serum, and in vivo stress conditions, construct USP mutants, and determine mutants’ role in E. ictaluri virulence. We found that usp05, usp07 and usp13 genes were highly expressed under all stress conditions, while groEL, groES, dnaK, grpE, and clpB were highly expressed in oxidative stress. Among the 10 E. ictaluri USP mutants, Eiusp05-07-08-09-10, and 13 were significantly attenuated in catfish and highly protective against wild type E. ictaluri infections in catfish. Eiusp05-07-08-09, and 13 were sensitive to oxidative stress, and all mutants were sensitive to pH exposure.
URI
https://hdl.handle.net/11668/16645
Recommended Citation
Akgul, Ali, "The Role of Universal Stress Proteins in Edwardsiella ictaluri Virulence" (2017). Theses and Dissertations. 4646.
https://scholarsjunction.msstate.edu/td/4646