Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Nannapaneni, Ramakrishna
Committee Member
Haque, Zahur Z.
Committee Member
Sharma, Chander Shekhar
Date of Degree
5-9-2015
Document Type
Graduate Thesis - Open Access
Major
Food Science and Technology
Degree Name
Master of Science
College
College of Agriculture and Life Sciences
Department
Department of Food Science, Nutrition and Health Promotion
Abstract
Previous findings showed that Listeria monocytogenes exhibits higher heat tolerance to thermal exposure at 60°C when pre-exposed to sublethal heat stress at 48°C for 30/60 min. The objective of this study was to investigate the morphological changes that occurred in L. monocytogenes serotype 1/2a cells as visualized by transmission electron microscopy (TEM) after exposure to sublethal heat stress at 48°C for 60 min and in combination with lethal concentration of carvacrol for 30 min. The TEM micrographs revealed thickening of cell wall and cell membrane, and clumping of cytoplasm when subjected to sublethal heat stress followed by carvacrol treatment. These studies indicate that L. monocytogenes cells when adapted to heat stress may alter its morphology to protect themselves against carvacrol. Further studies will investigate the effect of increased contact time with carvacrol and with other essential oils on the ultrastructural changes in L. monocytogenes cells adapted to heat stress.
URI
https://hdl.handle.net/11668/19213
Recommended Citation
Saha, Sulagna, "Transmission Electron Microscopy Study of Listeria Monocytogenes Serotype 1/2A Cells Exposed to Sublethal Heat Stress and Carvacrol" (2015). Theses and Dissertations. 4778.
https://scholarsjunction.msstate.edu/td/4778
