Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Haque, Z. Zee
Committee Member
Tidwell, Diane K.
Committee Member
Williams, J. Byron
Committee Member
Silva, Juan L.
Committee Member
Nannapaneni, Ramakrishna
Date of Degree
8-11-2012
Document Type
Dissertation - Open Access
Major
Food Science and Technology
Degree Name
Doctor of Philosophy
College
College of Agriculture and Life Sciences
Department
Department of Food Science, Nutrition and Health Promotion
Abstract
Edam and Cheddar whey were thermized (T) for different time periods (5–30 min) at 70 degrees Celsius, vacuum evaporated, and spray dried to obtain whey powders (WP). Native and thermized WP were evaluated for antioxidative properties. Edam and Cheddar WP significantly reduced thiobarbituric acid-reactive substances (TBARS) and peroxide value (PV) in ferric-catalyzed soybean oil emulsions. Thermization significantly improved antioxidative properties of WP with 5T, 10T, and 15T treatments showing better protective effects compared to 20T and 30T treatments. Based on these results, native (0T) and thermized (5T, 10T, and 15T) Edam and Cheddar WP coatings, with and without enzymatically hydrolyzed casein (CH), were used to reduce oxidative degradation and moisture loss of cubed beef steak and pork loin. Thermized treatments significantly reduced TBARS and PV in both types of meat, with CH containing treatments showing enhanced antioxidative properties. Protein oxidation as reflected by carbonyl formation and a decrease in sulfhydryls was also reduced by thermization. Treated and native WP coatings significantly reduced moisture loss of meat samples. Cubed beef steak treated with Cheddar WP coatings were evaluated for microbiological quality and basic sensory attributes. Although not statistically significant, 5T, 10T, and 10TCH treatments numerically showed potential for microbial reduction up to 4 days of storage. Sensory evaluation showed significant protection of key sensory attributes by treatments throughout the storage period when compared to controls with the 5 min thermization showing the best effect on lowering offlavor development based on sensory scores. The effect of pH and glucomannan (GM) on antioxidative properties of thermized (5T) Cheddar WP coatings applied to catfish fillets was also investigated. Coatings at pH 7.0, 7.5, and 8.0 (with GM) showed markedly greater protection against oxidative degradation compared to coatings without GM, as reflected by TBARS values and PV after 7 days of storage. Carbonyl formation and oxidation of sulfhydryl groups were also significantly reduced by coatings containing GM. Cheddar WP-based edible coatings (pH 7.0–8.0) with GM markedly reduced lipid oxidation and protein oxidation of fresh catfish fillets.
URI
https://hdl.handle.net/11668/21055
Recommended Citation
Weerasinghe, Sashie, "Use Of Whey-Based Edible Coatings as Antioxidative and Antimicrobial Agents" (2012). Theses and Dissertations. 4881.
https://scholarsjunction.msstate.edu/td/4881